This course provides theoretical and practical background useful for engaging in chemical separations in chemistry, biology, clinical biochemistry, engineering, research, and industry. The course first introduces chemical separation principles, including the partition concept. This leads to a treatment of the theory gas chromatography followed by a description of GC instruments with an emphasis on detector technology. An analogous look at high performance liquid chromatography (HPLC) includes theory and basic instrument design with conventional detector technology. The combination of HPLC with various forms of mass spectrometry will then be covered, which will also involve applications of the technique in medical science. There will be a focus on other advanced separation techniques such as ion chromatography, supercritical fluid chromatography, conventional electrophoresis, capillary zone electrophoresis, and size exclusion chromatography.