Biology is in the midst of a 'systems' revolution, which emphasizes the evaluation of interactions between hundreds-thousands of biomolecules in parallel. This paradigm, which has spawned the ever-growing number of '–Omics' disciplines, has been driven in part by tools developed by analytical chemists (e.g., microfluidics, multiplexed separations, high-resolution spectroscopy). One analytical technique that has had a particularly important role in the revolution is mass spectrometry. In this course, we take a detailed look at mass spectrometry and associated methods, and survey the state-of-the-art in other analytical tools that are driving the -Omics revolution.