This course offers seven lectures in structural biology, bioinformatics and proteomics. While the methodological basis for the techniques will be covered, the emphasis will be on what structural biology, bioinformatics, and proteomics teach us about biology. The course will address protein structure and dynamics, structures of membrane proteins, and structures of supramolecular assemblies. You will learn how various research tools such as X-ray crystallography, NMR spectroscopy, and electron microscopy are used to determine atomic-resolution structures of biological macromolecules (proteins, nucleic acids, carbohydrates, and lipids), with a special emphasis on macromolecular complexes and assemblies. Bioinformatics and computational biology techniques to study genomic and proteome variations and their impact on biology will be introduced. The course will also focus on various applications of mass spectrometry-based proteomics, including the mapping of large-scale protein-protein interactomes and the global characterization of post-translational modifications in proteomes — advances that are revolutionizing our understanding of the molecular workings of biological systems.