Motivation/Objectives: A cell is the basic unit of life in all organisms. Understanding cellular structures and how cells function is fundamental to all aspects of biosciences and is the basis for disease diagnostics/therapeutics and drug discovery. For single cell studies, the development of enabling micro and nanoengineered techniques/systems is a highly active field. The objectives of this course are two folds: 1) The course targets engineering graduate students to introduce essential topics in cell biology. 2) The course will also discuss micro/nano fabricated/engineered techniques/systems for manipulating cells, stimulating cells, and quantitatively measuring cellular activities.