This course will provide a survey of experimental biophysical techniques. We will discuss methods that are used to examine both the structure and function of biophysical systems, from whole organisms down to single molecules. The course is intended both for students intending to work within an experimental setting and to help those with theoretical interests understand how their ideas may be validated. Topics may include laser spectroscopy, Forster resonance energy transfer (FRET), fluorescence correlation spectroscopy (FCS), confocal and multiphoton imaging, nonlinear microscopy, light sheet microscopy, super-resolved microscopy, scanning force microscopy, cryo-electron microscopy, optical and magnetic tweezers, force spectroscopy, structural characterization and molecular crystallography, nuclear magnetic resonance (NMR), as well as x-ray and neutron scattering.